Figure 4.

Genetic interactions between myb3r1/3/5 and ple-2 or ede1-1 reveal the functions of repressor MYBs in proliferating cells

1. Cytokinesis defects in ple-2 roots were partially suppressed by combinational mutations in MYB3R1/3/5. The roots of plants with the indicated genotypes were stained at 7 DAG by propidium iodide to visualize cell outlines and were analyzed by confocal microscopy. Magnified views of the boxed area are provided below each image, to show cytokinesis defects, such as gapped cell walls and cell wall stubs (indicated by asterisks).
2. Cytokinesis defects in ede1-1 cotyledons were partially suppressed by combinational mutations in MYB3R1/3/5. Cotyledons of plants (8 DAG) with the indicated genotypes were fixed, cleared, and observed by DIC microscopy. The ede1-1 mutation causes incomplete cytokinesis of guard mother cells, producing single-celled stomata (asterisk).

Data Information: WT, wild type; Δ3,5, myb3r3/5 double mutant; Δ1,3,5, myb3r1/3/5 triple mutant. Scale bars, 50 μm in (A), 20 μm in (B).