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. 2015 Jun 9;34(15):1992–2007. doi: 10.15252/embj.201490899

Figure 5.

Figure 5

Genome-wide identification of MYB3R3-bound genes in vivo

  1. MYB3R3-GFP associates with promoters of G2/M-specific genes. ChIP–qPCR assays were performed using whole seedlings of MYB3R3-GFP, and the results are shown as the percentage of DNA fragments co-immunoprecipitated with anti-GFP antibody relative to input DNA. Grey and black bars indicate the results from MYB3R3-GFP and wild-type seedlings, respectively. Each measurement was performed twice and produced similar results.
  2. Both G2/M-specific genes and E2F target genes are enriched in MYB3R3-bound chromatin regions. Venn diagram analysis was conducted to compare MYB3R3-bound genes with the indicated gene categories. There are significant overlaps between MYB3R3-bound and G2/M-specific genes, and between MYB3R3-bound and E2F target genes with P-value < 10−15 in Fisher’s exact test. Numbers represent the gene number in each category.
  3. Enrichment of the MSA and E2F motifs in MYB3R3-bound chromatin regions. Enriched sequences in immunoprecipitated DNA faction were searched using motif-finding software MEME-ChIP web tool (http://meme.nbcr.net/meme/tools/meme-chip).
  4. Peak distributions around CYCB1;2 and ORC1 genes in the ChIP-seq analysis of MYB3R3-GFP plants. CYCB1;2 and ORC1 genes are shown as the representative examples of G2/M-specific and E2F target genes, respectively.