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. 2015 Jun 9;34(15):1992–2007. doi: 10.15252/embj.201490899

Figure 6.

Figure 6

MYB3R3 and MYB3R4 both interact with RBR1 and differently associate with E2F isoforms

  • A, B MYB3R3-GFP and GFP-MYB3R4 both interact with RBR1 and CDKA;1, but with a different E2F isoform in Arabidopsis leaves. IP was performed with anti-GFP antibodies from protein extracts prepared from first leaf pairs of MYB3R3-GFP or GFP-MYB3R4 transgenic plants at indicated days after germination (DAG). In these transgenic plants, expression of GFP fusion proteins was driven by the corresponding native promoters. Co-IP of RBR1 and E2FB was examined by Western blot analyses using corresponding antibodies. For detection of MYB3R3-GFP and CDKA;1, anti-GFP and anti-PSTAIRE (specific to CDKA;1) antibodies were used. As input, 1/10 of IP was loaded. Coomassie staining of the same membrane was used as a loading control.
  • C MYB3R3-GFP interacts with E2FC, but GFP-MYB3R4 does not. IP was performed with anti-GFP antibodies from protein extracts prepared from first leaf pairs of MYB3R3-GFP or GFP-MYB3R4 transgenic plants at indicated days after germination (DAG). Co-IP of E2FC and CDKA;1 was examined by Western blot analyses using anti-E2FC and anti-PSTAIRE antibodies, respectively. As input, 1/16 of IP was loaded. Coomassie staining of the same membrane was used as a loading control.