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. 2015 Jul 1;34(15):2008–2024. doi: 10.15252/embj.201490252

Figure 3.

Figure 3

Post-transcriptional regulation of Myc protein expression by γc cytokine signalling

  • A Myc mRNA expression in CTL, generated as described in Materials and Methods, switched into decreasing concentrations of IL-2 for 2 h, shown relative to IL-2-deprived CTL (2 h) (n = 3, mean ± SEM).
  • B Myc mRNA expression in IL-2-maintained CTL treated with the indicated concentration of tofacitinib for 2 h, shown relative to untreated IL-2-maintained CTL (n = 3, mean ± SEM).
  • C Myc mRNA expression in CTL switched into IL-7 (5 ng/ml) or IL-15 (20 ng/ml) or maintained in IL-2 (20 ng/ml) for 18 h, shown relative to IL-2-maintained CTL (n = 3, mean ± SEM).
  • D, E Myc-IRES-GFP-transduced CD8+ cells were sorted by FACS, and the Myc-IRES-GFPpos and Myc-IRES-GFPneg CTL were maintained in IL-2 (20 ng/ml) or deprived of IL-2 for 2 h. Data are representative of 3 experiments. (D) Western blot data show the expression of Myc, GFP and SMC1. (E) qPCR data show Myc mRNA expression relative to IL-2-maintained control (Myc-IRES-GFPneg) CTL (mean ± SEM, ANOVA used to determine statistical significance of multiple comparisons, ***P < 0.001).

Source data are available online for this figure.