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. 2015 Jun 24;34(15):2059–2077. doi: 10.15252/embj.201591125

Figure 4.

Figure 4

Altered SV exocytosis occurs at snapin-deficient presynaptic terminals

  • A Sample pHluorin images from synapses expressing VGluT-pHluorin-mCherry before and during a 10-Hz, 10-s stimulation (100 APs) in cortical neurons at DIV14. Exposure of pHluorin to the alkaline extracellular media during exocytosis gives rise to green fluorescence emission.
  • B, C Average pHluorin traces (B) and peak amplitudes (C) elicited by 20 (20 Hz, 1 s), 100 (10 Hz, 10 s), or 1,500 (5 Hz, 300 s) APs. Deleting snapin decreases total releasable pool size without affecting SVs recycling rate during a prolonged (300 s) stimulation. The total number of boutons analyzed is indicated within the columns. Data are means ± s.e.m., Student’s t-test. P-value: **** < 0.0001.