Fig. 8.

N-terminal charge of chimeric subunits from VLPs and detection of mRNAs. a N-terminal charges of CCMV-CP, NΔ24-CP, M2e(23)NΔ24-CP, VP1-CP, VP1-NΔ24-CP, 2C-CP, 2C-NΔ24-CP, HA-NΔ24-CP, CP-HA and CP-M2e(23) were calculated at pH 7.2 and 4.8 for ≥24 and ≥45 N- terminal residues using Protein Calculator version 3.3 (http://www.scripps.edu/~cdputnam/protcalc.html). b RT-PCR detection of mRNAs encoding CCMV coat proteins. ⊕Positive charge derived from NH₂ group. (+) indicates the presence of RNA followed by PCR product cloning and sequence confirmation and (-) for its absence not being cloned and sequenced