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. Author manuscript; available in PMC: 2016 Feb 11.
Published in final edited form as: Adv Funct Mater. 2014 Dec 28;25(6):977–986. doi: 10.1002/adfm.201403124

Figure 4.

Figure 4

Fluorescent micrographs comparing the effects of PD concentration and patterning on CM alignment and distribution. Figure A–F show epifluoresence images of day 7 CM fixed and stained with DAPI (blue) which stains the nuclei, and troponin I antibody (green) present in CM. Inserts in images D–F show higher magnification regions obtained via confocal microscopy showing cell-cell contact between adjacent channels. Figures G–L show confocal images of day 7 CM fixed and stained with DAPI (blue), connexin 43 (red) and sarcomeric α-actinin (green) which stains for the striated Z-lines of myotubes in cardiac muscle. Image plane is focused on cells at the top of the channels.