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Kinetic constants of Abl with ATP or ATP-dansyl as the cosubstrate.[a]
| cosubstrate | KM [μm] | kcat [s–1] | kcat/KM [s–1 mm–1] |
|---|---|---|---|
| ATP[b] | 51 ± 7 | 6.9 ± 0.8 | 130 |
| ATP-dansyl[b] | 115 ± 5 | 1.1 ± 0.1 | 9.6 |
| ATP-dansyl[c] | 39 ± 5 | 0.28 ± 0.10 | 7.2 |
Based on three independent trials with standard error shown.
Determined using the enzyme-coupled assay with peptide 5.
Determined using the FRET assay with ROX 5 peptide.
