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. Author manuscript; available in PMC: 2015 Aug 28.
Published in final edited form as: Nat Med. 2011 Dec 18;18(1):153–158. doi: 10.1038/nm.2558

Figure 2. SIRT1 preserves DARPP32 levels in medium spiny neurons, restores BDNF levels and facilitates TrkB activation in HD models.

Figure 2

(a-b) SIRT1 restored DARPP32 levels in N171-82Q HD mice (a) and BACHD mice (b). Top panels are representative Western blots and bottom panels are densitometry results. Mean ± S.E.M., n=4. (c-d) BDNF protein levels were measured by ELISA in cerebral cortex (c) or striatum (d) in 14-week-old mice. Mean ± S.E.M., n=3-6. (e) Representative Western blots of total Trk-B (t-TrkB) and phosphorylated TrkB (p-TrkB) levels in striatal cells expressing wild type (WT) huntingtin (SThdh Q7/Q7, WT) or mutant huntingtin (STHdh Q111/Q111, HD). Note that SIRT1 overexpression reduced mutant Htt-mediated dephosphorylation of Trk-B. (f) Quantification of p-TrkB levels from densitometry analysis of Western blots, Mean ± S.E.M., n=3. Top panel shows results as ratio of p-TrkB to β-actin; bottom panel shows results as ratio of p-TrkB to total TrkB (t-TrkB). (g) Quantification of total TrkB (t-TrkB) levels from densitometry analysis of Western blots, Mean ± S.E.M., n=3. *p<0.05, compared to the value of WT group; **p<0.05 compared to the value of HD group by Student’s t-tests.