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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Exp Eye Res. 2014 Dec 3;130:66–72. doi: 10.1016/j.exer.2014.12.002

Fig. 2.

Fig. 2

Tryptophan mutations have no effect on F5 secretion. (A) F5 domain organization and location of newly generated tryptophan residues, adapted from (Hulleman et al., 2012). (B) Conditioned media aliquots from transfected TREx-293 cells were taken 48 h after transfection and assayed for GLuc activity. (C) Western blot verification of GLuc activity levels shown in (B). n ≥ 3 independent experiments for all panels.