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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Exp Eye Res. 2014 Dec 3;130:66–72. doi: 10.1016/j.exer.2014.12.002

Fig. 3.

Fig. 3

Deletion of one or more insert regions in F3 does not increase F3 secretion to F5 levels or make it tolerant of tryptophan residues. (A) WT F5 is secreted more efficiently from TREx-293 cells than WT F3 under identical conditions. Absolute luciferase activity was measured 48 h after initial transfection. (B) Secretion of F3 deletion variants does not reach F5 levels. Single, double or triple deletion constructs were transfected into TREx-293 cells and 48 h afterward the amount of eGLuc2 was assayed in the conditioned media. F5 secretion levels 48 h after transfection are shown by a dotted line. (C) Western blot confirmation of (B). (D) Deletion variants do not have an increased tolerance for the pathogenic R345W mutation. Single, double or triple deletion constructs were generated in the R345W background and transfected into TREx-293 cells. 48 h afterward, the amount of eGLuc2 was assayed in the conditioned media. (E) Western blot confirmation of (D). n ≥ 3 independent experiments for all panels.