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. Author manuscript; available in PMC: 2015 Aug 28.
Published in final edited form as: J Gastroenterol Hepatol. 2013 Oct;28(10):1660–1668. doi: 10.1111/jgh.12291

Figure 4.

Figure 4

Chronic ethanol feeding and myriocin treatment effects on phosphorylated insulin, IGF and IRS signaling molecules in liver. Liver tissue from control and chronic ethanol-fed rats that were treated with vehicle or myriocin was used to measure (a) pYpY1162/1163-insulin receptor, (b) pYpY1135/1136-IGF-1 receptor, (c) pS312-IRS-1, (d) pT202/Y204-ERK1/2, (e) pS473-Akt, (f) pS9-GSK3b, (g) pT246-PRAS40, and (h) pT246pS112-p70S6K by multiplex or single-plex ELISA. Results normalized to protein content in the reactions. Box plots depict medians (horizontal bars), 95% confidence intervals (upper and lower limits of boxes), and ranges (stems). Inter-group comparisons were made by repeated measures one-way ANOVA with post-hoc Tukey tests of significance.