Table 1.

Primers designed using Primer Premier 6.0 software

Gene	Gene bank	Sequence
Type II collagen	NM_001006951.1	5′-GAAGAGCGGAGACTACTGGATTG-3′
		5′-AGGCGGAGGAAGGTCATCTG-3′
Type I collagen	NM_001003090.1	5′-TTCTGGTCCTCGTGGTCTCC-3′
		5′-CTTCACCGTCATCTCCGTTCTT-3′
Aggrecan	NM_001113455.1	5′-GCAGGACCAGACTGTCAGATAC-3′
		5′-TCCAGGCGTGTGATGAAGAAC-3′
SOX-9	NM_001002978.1	5′-CCAGCGAACGCACATCAAGA-3′
		5′-TGTAGGTGAAGGTGGAGTAGAGG-3′
GAPDH	NM_001003142.1	5′-TCTGCTCCTTCTGCTGAT-3′
		5′-GCCTGCTTCACTACCTTC-3′

Primer Premier 6.0 software from PREMIER Biosoft (palo alto, California, USA)

GAPDH glyceraldehyde-3-phosphate dehydrogenase, SOX-9 SRY-box 9