Fig. 2.

GVD and NFT progression in the hippocampal CA1 field. a Neurons in CA1 at 638× magnification showing various stages of GVD pathology: 1 a few granules in the neuronal cytoplasm staining weakly and one granule not at all for tau, 2, 3 more cytoplasmic granules develop and tau staining increases in intensity, 4 the cell body is completely filled with strongly staining small GVD and could be mistaken for NFT, 5 the neuron appears distended and the cell body is filled with large GVD with distended vacuoles. b, c, d CA1 neurons showing NFT progression at 638× magnification: i neurons showing a possible early stage of NFT formation with lightly staining PHF fibres, ii neurons showing robust tau staining of fibrous PHFs, iii ghost tangle. Scale bar = 20 μm. CA cornus ammonis, GVD granulovacuolar degeneration, NFT neurofibrillary tangles, PHF paired helical filament