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. Author manuscript; available in PMC: 2016 Aug 25.
Published in final edited form as: Cell Rep. 2015 Aug 13;12(8):1252–1260. doi: 10.1016/j.celrep.2015.07.039

Figure 2. Mutational Analysis Corroborates a C2:Kinase Domain Interface.

Figure 2

(A) Crystal structure of PKCβII (PDBID:3PFQ) showing predicted ion pairs between the kinase domain (cyan) or the C-terminal tail (gray) and the C2 domain (yellow).

(B–D) Normalized FRET ratio changes (mean ± SEM) representing PDBu- (200 nM) induced PKC translocation in COS7 cells co-expressing YFP-tagged PKCβII WT or mutants and plasma membrane-targeted CFP.

(E) FRET-ratio changes (mean ± SEM) representing thapsigargin- (5μM) followed by PDBu- (200 nM) induced PKC translocation in COS7 cells co-expressing YFP-tagged PKCβII WT or mutant and plasma membrane-targeted CFP.

See also Figure S1.