FIG 4.

TbSAS-6 RNAi impairs new flagellum assembly. (A) Immunostaining of 2N2K cells from noninduced control and 2N1K cells from TbSAS-6 RNAi cells (days 1 and 2) with L8C4 and YL 1/2 for flagellum and mature basal body, respectively. The white arrow showed a short new flagellum in a 2N1K cell with two mature basal bodies. Scale bar, 5 μm. (B). Quantitation of cells with different numbers of flagella and mature basal bodies in 2N1K cells from TbSAS-6 RNAi induced for 1 to 2 days and XN1K cells from TbSAS-6 RNAi induced for 2 days. About 200 cells were counted for each cell type, and the error bars represented SD calculated from three independent experiments. (C) Immunostaining of 1N1K cells from control and TbSAS-6 RNAi cells (2 days) with L8C4 and YL 1/2. Scale bar, 5 μm. (D) Quantitation of 1N1K cells with different numbers of mature basal bodies and flagella. About 200 1N1K cells were counted for each time point, and error bars represent SD calculated from three independent experiments. (E) Measurement of flagellum length and cell length of the 1N1K cells with one flagellum from control and TbSAS-6 RNAi (2 days) cells. About 120 1N1K cells from each sample were measured and plotted. Those circled within the dotted oval indicate the cells with a short flagellum.