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. 2015 Aug 27;14(9):898–907. doi: 10.1128/EC.00083-15

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FIG 6 — Overexpression of TbSAS-6 impairs the biogenesis of probasal body and flagellum. (A) Effect of TbSAS-6 overexpression on basal body duplication. Noninduced control and TbSAS-6 overexpression (day 1) cells were immunostained with 20H5 for both the mature basal body and probasal body (arrowheads) and with YL 1/2 for the mature basal body (arrows). Scale bar, 5 μm. (B) Quantitation of cells with different numbers of basal bodies. About 300 2N1K cells from TbSAS-6 overexpression cells and 200 2N2K cells from the noninduced control (Ctrl) cells were counted. Error bars represent SD calculated from three independent experiments. (C) Effect of TbSAS-6 overexpression on flagellum biogenesis. Noninduced control and TbSAS-6 overexpression (day 1) cells were immunostained with L8C4 for the flagellum and with YL 1/2 for the mature basal body (arrows). Scale bar, 5 μm. (D) Quantitation of cells with different numbers of flagella and mature basal bodies. About 300 cells from each cell type (2N1K cells from TbSAS-6 overexpression and 2N2K cells from the noninduced control) were counted. Error bars represent SD calculated from three independent experiments.