FIG 1.

Chitinolytic repertoire of U. maydis. (A) SMART (http://smart.embl-heidelberg.de/) domain architecture and SignalP prediction of U. maydis chitin-degrading enzymes. The U. maydis genome encodes three chitinases (GH-18 domain), Cts1, Cts2, Cts3, and one N-acetyl-glucosaminidase (GH-20 domain), Cts4. Cts1 lacks a conventional, N-terminal signal peptide (red). (B) The consensus sequence (gray) with the catalytic residues (black) characteristic for GH-18 and GH-20 glycosyl hydrolase are conserved (www.cazy.org). The experimentally verified active sites of S. cerevisiae Cts1 (ScCts1p; P29029) and Trichoderma harzianum NAG1 (ThNag1; P87258) are provided for comparison.