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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Transplantation. 2015 Sep;99(9):1817–1828. doi: 10.1097/TP.0000000000000718

Figure 6. B cell specific IL-10 is required for tolerance.

Figure 6

(A) MZP, MZ and CD19+CD5+CD1dhi+ B cells characterized from B-IL-10−/− or littermates by flow cytometry. IL-10 mRNA expression monitored by qRT-PCR. (B) Splenic B cell subsets (left), CD4+ and CD8+ cells subsets (upper right), and CD25 and Foxp3 subsets gated on CD4+ cells (lower right) analyzed. (C) B-IL-10−/− and littermate control recipients given tolerogen and BALB/c grafts. MZP B cells or follicular (Fol) B cells (4 X 105 cells/mouse) from wild-type or B-IL-10−/− naïve mice adoptively transferred on the day of transplant. Graft survival (upper) and parenchymal rejection score (lower). Littermate control or B-IL-10−/− + WT MZP B cells vs B-IL-10−/− mice (p<0.05); B-IL-10−/− vs B-IL-10−/− + WT Fol B cells or B-IL-10−/− + B-IL-10−/− MZP B cells (p ns). (D) Alloantibody responses of allograft recipients with the indicated genotypes and treatments measured 14 days after transplantation.