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ROP16 induced apoptosis and G1 arrest by phosphorylating p53 at Ser15/37 directly in NE-4C cells. NE-4C cells were infected with lentiviruce pCDH-p53wt/S15A/S37A/S15A37A and pCDH-ROP16/Flag before harvest. Cell cycle and apoptosis analysis of NE-4C cells were detected by flow cytometry for 3 times. Error bars showed mean ±SEM of at least 3 replicates (p<0.05).
