Fig 7. The feeding efficiency and survival of flies that had four bloodmeals on mice that received anti-recTSGF 1–2 antibodies.
(A) Mean engorged blood meal weights of flies measured before and after each blood meal. Eight cages of flies were allowed to feed on mice at Day 2, Day 5, Day 8 and Day 11 after the mice received rabbit anti-recTSGF treatment. Cages were weighed before and after each blood meal. Each data point corresponds to one cage, which includes the average blood engorgement data for all alive flies in that cage (n = 8 flies per cage at the beginning). (B) Percent survival of flies maintained on rec-TSGF antibody treated and control mice, respectively. The first day they were exposed to mice is considered Day 0. Both groups had 64 flies at the beginning of the experiment. (C) Average weight change in each cage between Day 1 and Day 12. Each data point represents the average weight change in each cage. As one mouse in the TSGF antibody treated group died before the 3rd blood meal, 6 cages of flies from the TSGF antibody treated group and 8 cages from the control group were measured here. (D): Lipid levels determined from flies 72 h after their 4th blood meal. Each data point represents the average of all alive flies in each cage (n = 6 cages in TSGF antibody treated group and n = 8 cages in control group). Statistical analyses were performed using the Mann Whitney test in the GraphPad Prism 6 software package. No significant difference were detected between the two groups (P>0.1).