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. 2015 Aug 28;3:71. doi: 10.3389/fped.2015.00071

Figure 2.

Figure 2

Effect of EPO treatment on HO activity and EPCs. (A) Heme oxygenase activity measured in whole lung lysates (methods). (B) Heme oxygenase mRNA expression, relative to 36B4 expression in whole lung. (C) Number of Endothelial Progenitor Cells (EPC) in blood at the day of sacrifice. (D) Example of fluorescence microscope picture of EPCs in blood. CON, control group, n = 4–8; PAH, untreated pulmonary arterial hypertension, n = 8–12; PAH + EPO, PAH treated with erythropoietin (EPO), n = 5–8. Differences between the PAH and CON groups were analyzed using t-tests for normally distributed data and Mann–Whitney U testing for not normally distributed data. Differences between the untreated and treated PAH groups (groups 1–4) were tested by one-way ANOVA followed by Fisher’s protected LSD post hoc testing (hematocrit, hemodynamics, RV hypertrophy, pulmonary vascular remodeling) and Kruskall–Wallis followed by Mann–Whitney post hoc testing with Bonferroni correction (number of EPCs) was used. *p < 0.05 vs. CON, #p < 0.05 vs. PAH.