Skip to main content
. 2015 Aug 28;6:430. doi: 10.3389/fimmu.2015.00430

Figure 3.

Figure 3

Phenotypic analysis of Tet+ T cells detected in spleen and lungs. Tet+ T cells detected in spleens and lungs of mice primed by SC route with CAF01 or by IN route with CpG and nasally boosted with CpG were characterized for the expression of transcription factors (A) and CXCR3 and CCR6 chemokine receptors (B,C). (A) Percentage of RORγt (filled bars) and T-bet (empty bars) positive cells among tetramer-binding CD4+ T cells detected in mice immunized with the vaccine combinations are reported in x axis. (B,C) Percentage (B) and mean fluorescent intensity [MFI, (C)] of CXCR3-positive (gray bars) and CCR6-positive cells (striped bars) among Tet+ T cells assessed in lungs of mice immunized with the vaccine combinations are reported in x axis. Bars represent the mean ± SEM of six mice per group and it is representative of two independent experiments. Statistical analysis was performed using the two-tailed Student’s t-test. *P < 0.05, **P < 0.01.