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. 2015 Jun 16;43(15):7280–7291. doi: 10.1093/nar/gkv597

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — (A) The Region I of σ⁵⁴ acts to inhibit RNA polymerase activity by blocking promoter DNA entry into the major DNA binding cleft. (B) Removal of Region I enables in vitro transcription from the late-melted nifH promoter (−10–1/WT) without the AAA+ activator PspF_1–275.