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Single-gene knock-out screening at 37°C yielded ttdR (encoding a nucleoid DNA-binding protein) as negatively impacting σ54 bypass transcription from the pspA promoter in vivo. In addition to increased bypass transcription from the ΦPpspAlacZ on XGal and MacConkey screens (Supplementary Table S2), the ΔttdR mutant also increased bypass transcription as measured in Miller Units. Experiments were performed with three biological replicates.
