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. 2015 Jul 7;43(15):7648–7660. doi: 10.1093/nar/gkv616

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Induction and growth kinetics for the high-copy glucarate (CdaR), erythromycin (MphR), acrylate (AcuR) and naringenin (TtgR) biosensors. Chemical inducers are added at time zero and fluorescence is observed for eight hours. Lower panels show the optical density of the induced cultures over time. Induction levels are indicated by shade, with darker colors indicating higher inducer concentrations. Glucarate induction levels are 40, 13, 4.4, 1.5, 0.49 mM and no inducer addition. Erythromycin induction levels are 1400, 450, 150, 51, 17 μM and no inducer addition. Acrylate induction levels are 5, 2.5, 1.3, 0.63, 0.31 mM and no inducer addition. Naringenin induction levels are 9, 3, 0.33, 0.11, 0.037 mM and no inducer addition. Fluorescence and optical density are normalized as described in the ‘Materials and Methods’ section. The standard error of the mean is represented with a 95% confidence interval (n = 3).