Figure 8.
Activation of the CdaR biosensor is well correlated with glucarate titers. Glucarate can be produced from myo-inositol by the enzymes MIOX and Udh. MIOX orthologs were transformed into cells containing Udh and the CdaR biosensor. Fluorescence was observed 48 h after addition of myo-inositol. Glucarate titers were measured after the same period of time in identical strains without the glucarate biosensor. All coefficients of variation are less than 10% (n = 3).