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. 2015 Jul 7;43(15):7398–7413. doi: 10.1093/nar/gkv692

Figure 2.

Figure 2.

DmSUV3 deficiency leads to a severe respiratory chain dysfunction. (A) Relative enzyme activities of respiratory chain enzyme complex I (NADH coenzyme Q reductase), complex I+III (NADH cytochrome c reductase), complex II (succinate dehydrogenase), complex II+III (succinate cytochrome c reductase) and complex IV (cytochrome c oxidase) in mitochondrial protein extracts from dmsuv3 KD (w;UAS-dmsuv3-RNAi/+;daGAL4/+) and control (w;UAS-dmsuv3-RNAi/+; and w;;daGAL4/+) larvae at 5 days ael. Data are represented as mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001, n = 5). (B) BN-PAGE analysis and in-gel staining of complex I and complex IV activities in mitochondrial protein extracts from control and dmsuv3 KD larvae at 5 days ael (CI, complex I; CIII, complex III; CIV, complex IV; SC, supercomplexes). (C) Western blot analysis of peptide steady-state levels of mitochondrial-encoded COX3 and nuclear-encoded NDUFS3 and ATP5A respiratory chain subunits in mitochondrial protein extracts from control and dmsuv3 KD larvae at 5 days ael. Porin was used as a loading control.