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. 2015 Jul 7;43(15):7398–7413. doi: 10.1093/nar/gkv692

Figure 4.

Figure 4.

dmsuv3 deficiency decreases mitochondrial tRNA steady-state levels and impairs mitochondrial translation. (A) De novo mitochondrial translation in isolated mitochondria from dmsuv3 KD (w;UAS-dmsuv3-RNAi/+;daGAL4/+) and control (w;UAS-dmsuv3-RNAi/+; and w;;daGAL4/+) larvae at 5 days ael. Loading was normalized to the total mitochondrial protein content per μl of sample. Coomassie Blue staining of the gel and porin Western blot of the input samples are shown as loading controls. (B) Northern blot analysis of the steady-state levels of mitochondrial tRNAs in KD and control larvae at 5 days ael. (C) Quantification of mitochondrial tRNA steady-state levels in control and KD larvae at 5 days ael. Loading of gels was normalized to 5S ribosomal RNA. (D) Schematic linearized map of D. melanogaster mtDNA.