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. 2015 Jul 7;43(15):7432–7446. doi: 10.1093/nar/gkv699

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Validation of structural requirement of the zipcode RNA for axonal localization. Panels (A)–(C) showed representative images of DRG neurons expressing GFP^myrzipcode^wild-type, GFP^myrzipcode^ΔACACCC, and GFP^myrzipcode^{U-rich replaced} constructs, respectively. GFP mRNA (red) and protein (blue) signals were observed in both the axons and growth cones (arrows) of DRG neurons transfected with reporter constructs containing the native-like secondary structure. However, those with the disrupted secondary structure [D; GFP^myrzipcode^{Δ(ACACCC/U-rich)}, E; GFP^myrzipcode^ΔU-rich] showed reporter mRNA in the cell body only (asterisks represented cell body in insets). Sense cRNA riboprobe for GFP showed no signal in exposure-matched images of GFP^myrzipcode^wild-type transfected DRGs (F).