Figure 3.

Thermal (UV-) melting profile for (A) pairing of XyloNA3 (xU12) with dA12; (B) pairing of XyloNA3 (xU12) with rA12. Sigmoidal melting curves are shown in different wavelengths (260, 284 and 295 nm). Heating- and cooling-curves are highlighted in red and blue respectively. Melting experiments were generally performed in 20 mM KH₂PO₄ buffer (pH 7.5) containing 0.1 M NaCl, and 0.1 mM Na₂EDTA or in a Mg²⁺-buffer, as indicated above, containing 10 mM Tris·HCl (pH 7.5) and 50 mM MgCl₂. Melting curves at 295 nm are from a faster heating-cooling experiments. Data presented here are only for a 1:1 mixing stoichiometry with 4 μM concentration of individual strand.