Table 1. Thermal melting data for the evaluation of self-pairing and cross-pairing abilities of XyloNA/dXyloNA vs. natural DNA/RNA systems.

Oligo. no.	Type of pairing	Sequence	Tm (°C)
ON-1	XyloNA1:XyloNA1	5′-x(GUGUACAC)-3′	67
		3′-(CACAUGUG)x-5′
ON-2a	dXyloNA:dXyloNA	5′-dx(GTGTACAC)-3′	57
		3′-(CACATGTG)dx-5′
ON-3	RNA:RNA	5′-r(GUGUACAC)-3′	41
		3′-(CACAUGUG)r-5′
ON-4a	DNA:DNA	5′-d(GTGTACAC)-3′	26
		3′-(CACATGTG)d-5′
ON-5	XyloNA2:RNA	5′-x(UGCUACGC)-3′	n.o.
		3′-(ACGAUGCG)r-5′
ON-6	XyloNA2:DNA	5′-x(UGCUACGC)-3′	n.o.
		3′-(ACGATGCG)d-5′
ON-7	RNA:RNA	5′-r(UGCUACGC)-3′	50
		3′-(ACGAUGCG)r-5′
ON-8	RNA:DNA	5′-r(UGCUACGC)-3′	n.c.
		3′-(ACGATGCG)d-5′
ON-9	XyloNA3:RNA	xU12:rA12	37–50
ON-10	XyloNA3:DNA	xU12:dA12	35–38

Melting points were determined in a buffer solution (pH 7.5) containing 0.1 M NaCl, 20 mM KH₂PO₄, and 0.1 mM Na₂EDTA. For the self-complementary sequences (ON-1 to ON-4) 8 μM and for other sequences (ON-5 to ON-10) 4 μM concentration of each strand have been used.

[a] Ref. (24); n.o., no cross-pairing observed; n.c., T_m could not be calculated due to an unfavorable melting profile. Melting profiles for ON-3, ON-7, and ON-8 are provided in the SI (Supplementary Figure S2–S4).