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. 2015 Jul 24;43(15):7480–7488. doi: 10.1093/nar/gkv734

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — RGVG M6 can transcribe fully-modified RNA. Transcription assay for RGVG-M6 catalyzed incorporation of ribonucleotides (rN); 2′-O-methyluridine (rVmU); 2′-O-methylpyrimidines (rRmY); 2′-O-methyladenosine and 2′-O-methylpyrimidines (rGmH); 2′-O-methylnucleotides (mN); 2′-fluoro-purines and 2′-O-methylpyrimidines (fRmY); and 2′-fluoro-guanosine, 2′-O-methyladenosine and 2′-O-methylpyrimidines (fGmH). Transcripts were analyzed by denaturing PAGE and imaged after staining in SYBR-Gold. Transcriptions ran 20 h. A reaction (10-fold diluted) containing WT T7 RNA polymerase with ribonucleotides (rN) is shown for comparison.