Figure 2.

Discordant regulation of ΔNp63 and miR-944 expression under various conditions. (A) Human primary keratinocytes were differentiated by cultivating for the indicated time period after the replacement of culture medium with a medium containing a high calcium concentration (1.2 mM). The relative expression levels of ΔNp63 and miR-944 (left panel) and the expression of precursor TP63 mRNA (right panel) were examined during calcium-induced keratinocyte differentiation. Data represent the means ± SD of triplicate biological samples and are representative of four different experiments. *P < 0.05 versus semi-confluence, unpaired Student's t-test. (B) Human primary keratinocytes were treated with the indicated concentration of TSA for 4 days. The relative expression levels of ΔNp63 and miR-944 (left panel) and the expression of precursor TP63 mRNA (right panel) were examined. Data represent the means ± SD of triplicate biological samples and are representative of three different experiments. *P < 0.05 versus TSA 0 μM, unpaired Student's t-test. (C) Box plots showing normalized gene expression levels of TP63 and MIR944 in the skin of healthy individuals and in the lesional and non-lesional skin of psoriasis patients, which were examined using GEO deposited data (TP63 expression, GEO accession number: GSE14905; miR-944 expression, GEO accession number: 31037). (D) Human primary keratinocytes were exposed to 30 mJ/cm² of UVB daily for 3 consecutive days. The relative expression levels of ΔNp63 and miR-944 (left panel) and the expression of precursor TP63 mRNA (right panel) were examined. Data represent the means ± SD of triplicate biological samples and are representative of three different experiments. *P < 0.05 versus control, unpaired Student's t-test.