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The different conformations of reactant and product in the active site. (A) The structure alignment of Sub-U and Sub-Ψ and their orientation relative to D85. (B) The distances between Cγ atoms of D85 and the mass center of Sub-U (black) and Sub-Ψ (red). (C) Two water molecules penetrate into the active site and form hydrogen bonds with Ψ and the sugar phosphate in the preceding residue.
