TABLE 1 .

Dissociation constants of WT RctB and RctB ΔC159 for various DNA fragments

DNA probe	Avg Kd (nM) ± SDa of:
	WT RctB	RctB ΔC159
12×6 Dam+	0.0227 ± 0.0198	0.0094 ± 0.004
12×6 Dam−	NDb	13.84 ± 3.86
Inc 11	197.6 ± 252.3	0.061 ± 0.11
Inc 12	2.98 ± 0.70	0.00016 ± 0.00025
11×6	8.50 ± 6.92	5.45 ± 2.87
11+1×6	7.85 ± 8.13	5.35 ± 6.25
Rev12×6	0.22 ± 0.16	0.29 ± 0.20
12×2	10.16 ± 7.14	0.26 ± 0.029
12×4	0.023 ± 0.0071	0.036 ± 0.029
12-1	0.036 ± 0.026	0.0081 ± 0.0025
12-2	0.092 ± 0.11	0.012 ± 0.06
12-3	0.06 ± 0.015	0.032 ± 0.0025
12-4	0.11 ± 0.021	0.04 ± 0.029
12-5	0.087 ± 0.03	0.038 ± 0.025
12-6	0.093 ± 0.013	0.17 ± 0.033
12-135	1.07 ± 0.23	0.35 ± 0.59
12-246	2.13 ± 0.40	1.20 ± 0.96
12-12456	42.62 ± 22.23	5.66 ± 2.97

^aAverage values and standard deviations were obtained by quantitation of at least three independent binding experiments.

^bND, not determined.