FIG 3 .

Morphological defects in S. aureus cells lacking glucosaminidases. (a) Images of fixed, Van-FL-labeled cells showing altered morphology. Arrowheads indicate hemispherical cells. Cells of this shape are not found in wild-type populations unless attached to a sister cell. (b) Examples of hemispherical cells. In some cells, septa are visible in bacteria that have not completed the shape change to the mature spherical morphology. This shows that correct shape change is not taking place within the duration of the cell cycle. (c) Quantification of the proportion of hemispherical cells in each sample. P values are the result of Fisher’s exact tests comparing the wild type with each mutant.