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. 2015 Aug 28;5:13519. doi: 10.1038/srep13519

Figure 5. Conversion of insulin signaling in GADD34-deficient mice by aging and HFD feeding.

Figure 5

(a) Addipocytes were proliferated and differentiated from WT and GADD34-deficient MEFs by insulin treatment. Picture shows the Oil Red O staining. The numbers of Oil red O positive cells shown are the mean ratio ± SEM. (Tree experiments *p < 0.05). (b) WT and GADD34-deficient MEFs were treated with insulin (10 μg/ml). Protein expression of p-Akt, Akt and GAPDH were analyzed by western blotting. The original immunoblots are presented in Supplemantary Figure 10c. (c) Three months old WT and GADD34-deficient mice were fed ND and HFD for 2 weeks. After 5 h starvation, insulin (5 U/mice) were injected intravenously. The livers were removed from these mice in each time (0 min, 3 min, 15 min). Protein expression of p-Akt, Akt and actin in the liver were analyzed by western blotting. The original immunoblots are presented in Supplemantary Figure 10d.