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. 2015 Aug 28;5:13519. doi: 10.1038/srep13519

Figure 6. Palmitate induces phosphorylation of JNK and inhibits insulin signaling.

Figure 6

WT and GADD34-deficient MEFs were treated with BSA (0.17 mM) or palmitate-BSA (0.2 mM/0.17 mM). (a) Protein expression of GADD34 were analyzed by western blotting. (b) Expression of TNF-α mRNA and production of TNF-α in MEF stimulated with BSA or BSA-palmitate. (c) Protein expression of p-JNK (JNK and β-actin as control) were analyzed by western blotting. The original immunoblots are presented in Supplemantary Figure 10d. (d) WT and GADD34-deficient MEFs were stimulated with insulin (10 μg/ml) after BSA (0.17 mM) or palmitate-BSA (0.2 mM/0.17M) treatment for 24 hours. Protein expression of p-Akt, p-JNK and β-actin at 0, 15 min or 2h were analyzed by western blotting. The original immunoblots are presented in Supplemantary Figure 10e.