Table 1. Description of inter-assay reproducibility, reproducibility with processing delays, and short-term within-person stability experiments.
Experiment | Inter-assay reproducibility | Reproducibility with processing delays | Short-term within-person reproducibility |
Participants | HPFS | Non-HPFS | HPFS |
N | 15 | 12 | 80 (40 healthy and 40 less healthy*) |
Sample | EDTA plasma | EDTA plasma | EDTA plasma |
Number of aliquots | 2 | 3 | 2 |
Timing | (1) Processed immediately | Donated mean 9.4 | |
Split sample | (2) Stored 24 h at 4°C | (range 6.5 to 12.9) | |
(3) Stored 48 h at 4°C | months apart | ||
Storage conditions | Liquid nitrogen (below -150°C) | Liquid nitrogen (below -150°C) | Liquid nitrogen (below -150°C) |
for 1–2 years | for 4–5 years | for 13–14 years |
HPFS = Health Professionals Follow-up Study
* As indicated by smoking status, age (≤ or > 65 years), BMI (normal weight/overweight), and/or hypertensive state.