Table 3. Average and maximum pair CVs for standardized values of 61 miRNAs measured in 15 duplicate (split) samples.
| Expressed at detectable levels in > 50% of samples | Expressed at detectable levels in ≤ 50% of samples | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Target meets established criteria | miRNA | Avg CV | Maximum CV | % Detected | Target meets established criteria | miRNA | Avg CV | Maximum CV | % Detected |
| Yes | hsa-miR-20b-5p | 2.6 | 7.3 | 50% | Yes | hsa-miR-26b-3p | 2.4 | 6.2 | 3% |
| Yes | hsa-miR-29b-3p | 3.8 | 8.8 | 100% | Yes | hsa-miR-29a-3p | 2.2 | 6.2 | 3% |
| Yes | hsa-miR-199a-3p | 3.5 | 9.9 | 87% | Yes | hsa-miR-223-5p | 2.9 | 13.1 | 3% |
| Yes | hsa-miR-26b-5p | 4.0 | 16.6 | 60% | Yes | hsa-miR-145-5p | 2.0 | 6.2 | 10% |
| Yes | hsa-miR-375 | 5.7 | 14.9 | 53% | Yes | hsa-miR-130b-3p | 2.5 | 7.9 | 7% |
| Yes | hsa-miR-93-5p | 4.3 | 16.5 | 90% | Yes | hsa-miR-140-3p | 3.0 | 7.2 | 33% |
| Yes | hsa-miR-21-5p | 5.5 | 19.0 | 80% | Yes | hsa-miR-222-3p | 2.2 | 6.6 | 40% |
| Yes | hsa-miR-320a | 7.1 | 19.0 | 87% | Yes | hsa-miR-122-5p | 4.1 | 15.3 | 13% |
| Yes | hsa-miR-27b-3p | 8.0 | 40.1 | 100% | Yes | hsa-miR-185-5p | 3.2 | 11.0 | 20% |
| Yes | hsa-miR-17-5p | 6.6 | 19.9 | 67% | Yes | hsa-let-7d-5p | 2.9 | 11.8 | 40% |
| Yes | hsa-miR-30b-5p | 8.5 | 41.7 | 93% | Yes | hsa-miR-126-5p | 4.8 | 22.0 | 10% |
| Yes | hsa-miR-126-3p | 6.3 | 34.0 | 93% | Yes | hsa-miR-133a | 3.4 | 10.1 | 27% |
| Yes | hsa-miR-106b-5p | 7.6 | 30.7 | 93% | Yes | hsa-miR-424-5p | 7.9 | 94.2 | 3% |
| Yes | hsa-miR-130a-3p | 7.7 | 29.1 | 90% | Yes | hsa-miR-23b-5p | 8.0 | 91.7 | 3% |
| Yes | hsa-miR-27a-3p | 8.4 | 27.1 | 93% | Yes | hsa-miR-424-3p | 8.3 | 94.2 | 3% |
| Yes | hsa-miR-191-5p | 8.1 | 34.7 | 93% | Yes | hsa-miR-155-5p | 9.8 | 80.0 | 7% |
| Yes | hsa-miR-30c-5p | 9.3 | 40.1 | 100% | No | hsa-miR-22-5p | 23.7 | 125.8 | 10% |
| Yes | hsa-miR-26a-5p | 8.2 | 29.5 | 93% | — | hsa-miR-151a-3p | NC | NC | 0% |
| Yes | hsa-miR-221-3p | 9.3 | 32.8 | 93% | — | hsa-miR-151a-5p | NC | NC | 0% |
| Yes | hsa-miR-30e-3p | 13.9 | 45.9 | 100% | — | hsa-miR-191-3p | NC | NC | 0% |
| Yes | hsa-miR-409-3p | 11.6 | 31.7 | 70% | — | hsa-miR-22-3p | NC | NC | 0% |
| Yes | hsa-miR-320b | 18.6 | 83.1 | 100% | — | hsa-miR-193b-3p | NC | NC | 0% |
| Yes | hsa-miR-92a-3p | 11.9 | 62.2 | 93% | — | hsa-miR-199a-5p | NC | NC | 0% |
| No | hsa-miR-29c-3p | 20.7 | 58.1 | 100% | — | hsa-let-7e-5p | NC | NC | 0% |
| — | hsa-miR-210 | NC | NC | 0% | |||||
| — | hsa-miR-361-5p | NC | NC | 0% | |||||
| — | hsa-miR-142-5p | NC | NC | 0% | |||||
| — | hsa-miR-23b-3p | NC | NC | 0% | |||||
| — | hsa-miR-1 | NC | NC | 0% | |||||
| — | hsa-miR-25-3p | NC | NC | 0% | |||||
| — | hsa-let-7d-3p | NC | NC | 0% | |||||
| — | hsa-miR-10a-5p | NC | NC | 0% | |||||
| — | hsa-miR-130b-5p | NC | NC | 0% | |||||
| — | hsa-miR-133b | NC | NC | 0% | |||||
| — | hsa-miR-145-3p | NC | NC | 0% | |||||
| — | hsa-miR-29c-5p | NC | NC | 0% | |||||
| — | hsa-miR-93-3p | NC | NC | 0% | |||||
Bold indicates acceptable results and italics indicates unacceptable results. Avg CV = average CV; Max CV = maximum CV; % Detected = % samples with detectable levels; NC = not calculated.