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. 2015 Aug 27;10(8):e0135278. doi: 10.1371/journal.pone.0135278

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© 2015 Ullman et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 7 — A) B11 internalization into early endosomes was followed by co-localization experiments using an anti-FLAG antibody (green) and the EEA1 protein, a marker of early endosome compartment, and imaged with the anti-EEA1 antibody (red). As shown in the merged images the two proteins co-localized (yellow) within the early endosome vesicles at 30 minutes. B) B11 trafficking into the lysosomes was followed by co-localization experiments using the anti-FLAG antibody (green) and anti-LAMP1 (red). Co-localization can be observed in the merged view (yellow) at 60 minutes.