Table 1. Naturally induced anti-PfMSP6 antibodies predominantly target the N-terminal domain.

Responses against PfMSP6 antigens in HB3-infected (n=309, Table 1A) and K1-infected (n=33, Table 1B) individuals. IgG antibody level [µg/ml] were calculated as described in Materials and Methods, and responses scored as positive (% Positive) if they exceeded the mean plus three standard deviations of the antigen-specific responses in a pool of control serum from non-P. falciparum infected individuals. Minimum (Min), Lower Quartile (LQ), Median, Upper Quartile (UQ) and Maximum (Max) values were generated for all responders.

A			Total IgG Antibody Levels (ug/ml)
Antigen	Cut-off (ug/ml)	% Positive	Min	LQ	Median	UQ	Max
HB3 N-Term	0.492	40.1	0.191	0.271	0.400	0.718	319.786
K1 N-Term	0.370	37.2	0.188	0.237	0.319	0.459	48.307
C-Term	0.550	23.0	0.203	0.249	0.285	0.468	15.403

B			Total IgG Antibody Levels (ug/ml)
Antigen	Cut-off (ug/ml)	% Positive	Min	LQ	Median	UQ	Max
HB3 N-Term	0.492	48.5	0.199	0.307	0.442	1.440	60.317
K1 N-Term	0.370	45.5	0.206	0.241	0.341	0.627	10.659
C-Term	0.550	24.2	0.203	0.233	0.281	0.400	5.001