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. Author manuscript; available in PMC: 2015 Aug 28.
Published in final edited form as: Chem Commun (Camb). 2015 Feb 21;51(15):3266–3269. doi: 10.1039/c4cc09765c

Fig. 1.

Fig. 1

Fluorescence images of sinusoidal microchannels (A) before and (B) after 5 min enzymatic cleavage of a 3′-Cy3 modified 25 nt oligonucleotide containing a dU residue. The microchannel surfaces were activated using UV/O3 exposure and functionalized with 20 mg mL−1 EDC and 2 mg mL−1 NHS in 0.1 M MES (pH 4.8). After 20 min of the EDC–NHS reaction, a 40 µM solution containing the 3′-Cy3 oligonucleotide was infused into the device. Before imaging, the device was rinsed with 0.1% SDS and nuclease-free water.