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HD HDL shifts toward the smaller HDL3 subclass. HDL (5 µg protein/lane) was separated by native gradient gel electrophoresis and stained with Coomassie Brilliant Blue. Intensity blots of individual samples were obtained, and the peak areas of HDL2 and HDL3 were calculated. Total peak area was set to 100% and used to calculate the percentage of HDL2/HDL3. Values shown represent means±SEMs of three individual experiments. CON, control.
