Figure 2.

Cholesterol efflux capability is impaired in HDL from patients on HD or PD. (A) HDL levels from 24 patients on HD, 14 patients on PD, and 20 control subjects (CON) were examined for their ability to efflux [³H]-cholesterol from TO-901317–stimulated, lipid-loaded RAW 264.7 macrophages. [³H]-cholesterol–labeled cells were incubated with HDL (50 µg/ml) for 2 hours at 37°C. Cholesterol efflux is expressed as the radioactivity in the medium relative to total radioactivity in medium and cells. (B) HDL was labeled with DiI and examined for its ability to promote lipid uptake by HepG2 cells. Cellular uptake of the fluorescent lipophilic dye DiI was quantified by flow cytometry. Values shown represent means of three independent experiments.