Large glomeruli from adults contained more podocytes, NECs and PECs than glomeruli from children. A and B show representative confocal images of glomeruli from children and adults, respectively, with WT-1 (green), vWF (red), and DAPI (blue). C and D show the numbers of NECs, PECs, and podocytes per glomerulus from children and adults. C illustrates the variability of each cell type between glomeruli from adults and children; each circle represents the value for a single glomerulus,. D provides details of the medians, IQRs (25% and 75% percentiles), minimum and maximum values, and fold ranges per group. Scale bars, 100 μm. DAPI, 4′,6-diamidino-2-phenylindole. **P<0.01; ***P<0.001.