Bone marrow analysis by flow cytometry. (A and B) Quantitation of progenitor cells within bone marrow (A) and quantitation of bone marrow cells with differentiation markers (B), as described in the Concise Methods, from THP+/+ and THP−/− mice (n=5 each group). The Mϕ/DC population shown is CD11b+ and Ly6G−. The decrease in granulocyte progenitors and increase in neutrophils strongly support enhanced granulopoiesis. (C) ELISA assay for G-CSF, performed on sera from THP+/+ and THP−/− mice (n=5 each group). Asterisk denotes statistical significance between the two strains. LT-HSC, long-term hematopoietic stem cell; ST-HSC, short-term hematopoietic stem cell; MPP, multipotent progenitor; CLP, common lymphoid progenitor; CMP, common myeloid progenitor; GMP, granulocyte-macrophage progenitor.