Fig. 3.

A PfGEXP5-GFP chimera is exported to the RBC cytoplasm. a Western blot analysis of the 3D7 parent line and the PfGEXP5-GFP transfectant. Stage III gametocytes were treated with Eqt-II and the pellet (P) and supernatant (SN) fractions subjected to Western blot and probed with anti-PfGEXP5 and anti-GFP antibodies. b Expression of PfGEXP5-GFP at different stages of gametocyte development; GFP fluorescence signal and DIC images (BF). The GFP fluorescence is present in the RBC cytoplasm. Arrows in the top panel indicate accumulation of PfGEXP5-GFP at the PV. Scale bars 5 μm. c Immunofluorescence microscopy of sexually induced PfGEXP5-GFP transfectants (14 h pi), probed with rabbit anti-GFP (green) and mouse anti-Pfs16 (red) antibodies. d Immunofluorescence microscopy of sexually induced PfGEXP5-GFP transfectants (14 h pi, top) and an asexual trophozoite from the same line (bottom), probed with anti-GFP (green) and anti-KAHRP (red) antibodies. Scale bars 3 μm