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. 2015 Aug 27;10(8):e0136652. doi: 10.1371/journal.pone.0136652

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© 2015 Pietrzyk-Nivau et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — (A) Histogram representation of the expression levels of various genes determined by RT-qPCR in 3D (black bars) and liquid-culture (white bars) cells at day 9. (B) Histogram representation of time-dependent expression levels of various genes determined by RT-qPCR in 3D cells at days 9 (white bars), 16 (gray bars) and 23 (white bars). mRNA percentage of each gene was normalized to that of HPRT mRNA percentage and calibrated via the ΔΔCt method [27]. Data are means ± SEM of 3 independent experiments.*p<0.05. In 3D, days 16 and 23 were compared to day 9. Commercial references of the primer mixes used for qPCR are detailed in S1 Table.